15-Hydroxylation of 5 beta-cholestan-3 alpha-ol and 24 alpha-ethyl-5 beta-cholestan-3 alpha-ol in rat liver supernatants (18,000 X g).

Conversion of Sa-Cholestan-3@-ol to ~‘-5~-Cholesten-3~-ol in Cockroaches*

Detailed studies of the sterol requirement of the carnivorous beetle Dermestes vulpinus (1) which have been carried out in this laboratory have indicated that up to 97% of the normal dietary cholesterol requirement of this insect may be replaced by a number of other sterols. One of the compounds capable of sparing but not completely replacing cholesterol in the diet of these insects is cholesta...

Inhibitors of ergosterol biosynthesis and growth of the trypanosomatid protozoan Crithidia fasciculata.

Six nitrogen-, sulfur- and cyclopropane-containing derivatives of cholestanol were examined as inhibitors of growth and sterol biosynthesis in the trypanosomatid protozoan Crithidia fasciculata. The concentrations of inhibitors in the culture medium required for 50% inhibition of growth were 0.32 microM for 24-thia-5 alpha,20 xi-cholestan-3 beta-ol (2), 0.009 microM for 24-methyl-24-aza-5 alpha...

Appearance of atypical 3 alpha,6 beta,7 beta,12 alpha-tetrahydroxy-5 beta-cholan-24-oic acid in spgp knockout mice.

Bile formation and its canalicular secretion are essential functions of the mammalian liver. The sister-of-p-glycoprotein (spgp) gene was shown to encode the canalicular bile salt export protein, and mutations in spgp gene were identified as the cause of progressive familial intrahepatic cholestasis type 2. However, target inactivation of spgp gene in mice results in nonprogressive but persiste...

Integrin alpha v beta 3 differentially regulates adhesive and phagocytic functions of the fibronectin receptor alpha 5 beta 1

The plasma protein fibronectin is an important opsonin in wound repair and host defense. To better understand the process of fibronectin-mediated phagocytosis, we have transfected K562 cells, which endogenously express alpha 5 beta 1, with alpha v beta 3. In these transfectants, antibodies to alpha v beta 3 block phagocytosis of fibronectin-opsonized beads completely, even though half the inges...

Metabolism of Coprostanol-c14 and Cholestanol-4-c14 in Man.